PMPM Pronuclear Microinjection services produce transgenic founders by injection of linearized DNA directly into the pronuclei of a zygote. DNA constructs provided by the researcher must be prepared following specific PMPM protocols.
Pronuclear Microinjection into B6C3 zygotes per 100 viable embryos $2,950
Pronuclear Microinjection into C57BL/6 zygotes per 100 viable embryos $3,250
Note: Pronuclear Microinjection service includes superovulation, mating and harvesting of donor embryos, microinjection, and surgical transfer of a minimum of 100 viable embryos. Animal costs per round of microinjection are included in service fee.
PMPM ESC Microinjection services provide many options for your gene targeting needs. Service options are completely customizable to suit your specific project.
Clone expansion per clone $400
typical gene targeting project utilizes 2 clones
ESC preparation for microinjection per clone $140
Morula microinjection per clone $2,975
fee includes embryo donors and pseudopregnant recipients
Test breeding of germline transmission per breeding pair $75
varies based on number of chimeras generated