Research Summary

My lab is interested in understanding the cell signaling of specific membrane receptors, combining aspects of Cell Biology, Immunology , Biochemistry and Molecular Biology.

Structure and function of the antigen receptor on B lymphocytes:
The B cell receptor is composed of membrane immunoglobulin M (mIgM) and a heterodimer of two associated proteins, Ig-alpha and Ig- beta. When the receptor is on the cell surface of B lymphocytes it functions to transmit intracellular signals that regulate cell growth and differentiation and it binds to antigen for the generation of the immune response. We are studying the downstream consequences of intracellular signaling and how the BCR interacts with protein tyrosine kinases, phosphatases and other associated proteins. In addition we are exploring how signaling changes the cytoskeletal architecture in B lymphocytes. Recently my lab has embarked on a study of the gap junction protein connexin43 (Cx43), whose expression profoundly influences lymphocyte responses to BCR and chemokine receptor signaling.

Bio

A.B., Barnard College
M.A., M.Phil, Ph.D. Columbia University (New York)
Postdoctoral Fellow, University of California Berkeley (1981-1985)
Assistant Research Biochemist, University of California San Francisco (1985-1991)
UBC since 1992
Associate Director of the Life Sciences Institute (2008-2013)

Publications
  1. Falk, L., M. Dang-Lawson, J.L. Vega, F. Pournia, K. Choi, C. Jang, C.C. Naus, L. Matsuuchi. 2014. Mutations of Cx43 that affect B cell spreading in response to BCR signalling. Biology Open 3: 185-194
  2. Machtaler, S., K. Choi, M. Dang-Lawson, L. Falk, F. Pournia, C.C. Naus, L. Matsuuchi. 2014. The role of the gap junction protein connexin43 in B lymphocyte motility and migration. FEBS Letters 588: 1249-1258
  3. Matsuuchi, L. and C.C. Naus. 2013. Gap junction proteins on the move: Connexins, the cytoskeleton and migration. Biochimica et Biophysica Acta 1828: 94-108
  4. Machtaler, S., Dang-Lawson, M., Choi, K., Jang, C., Naus, C.C. and L. Matsuuchi. 2011. The Gap junction protein Connexin43 regulates B-lymphocyte spreading and adhesion. Journal of Cell Science 124: 2611-2621
  5. Jang, C., Machtaler, S. and L. Matsuuchi. 2010. The role of Ig-α and Ig-β in B cell antigen receptor internalization. Immunology Letters 134: 75-82
  6. Price, PW, McKinney, EC, Wang, Y, Sasser, LE, Kandasamy, MK, Matsuuchi, L, Milcarek, C, Deal, Meagher, RB. 2009. Engineered cell surface expression of membrane immunoglobulin as a means to identify monoclonal antibody-secreting hybridomas. J. Immunol. Methods 343: 28-41
  7. Dylke, J., Lopes, J., Dang-Lawson, M., Machtaler, S. and Matsuuchi, L. 2007. Role of the extracellular and transmembrane domains of Ig-α/β in assembly of the B cell antigen receptor. Immunology Letters 112: 47 – 57
  8. Jackson, T.L., Santos, L., Dang-Lawson, M. and Matsuuchi, L. 2005. Localization of the Ig α/β component of the BCR in membrane microdomains in a B lymphoma variant. Immunology Letters 99: 69-79
  9. Wang, L.D., Lopes, J., Cooper, A.B., Dang-Lawson, M., Matsuuchi, L. and M.R. Clark. 2004. Selection of B lymphocytes in the periphery is determined by the functional capacity of the B cell antigen receptor. PNAS 101: 1027-1032
  10. Foy, S.P. and Matsuuchi, L. 2001. Association of chains of the B cell antigen receptor with multiple proteinchaperones. Immunology Letters. 78: 149-160
  11. Ingham, R., Santos, L., Dang-Lawson, M., Holgado-Madruga, M., Dudek, P., Maroun, C., Wong, A.J., Matsuuchi, L. and Gold, M.R. 2001. The Gab1 docking protein links the B cell antigen receptor to the phosphatidylinositol 3-kinase/Akt signaling pathway and to the SHP-2 tyrosine phosphatase. Journal of Biological Chemistry 276: 12257 — 12265
  12. Matsuuchi, L.* and Gold, M.R. 2001. New views of B cell antigen receptor structure and organization. Current Opinion in Immunology (June) 13: 270-277 (*corresp. auth)
  13. Condon, C., Hourihane, S.L., Dang-Lawson, M., Escribano, J.M. and Matsuuchi, L. 2000. Aberrant trafficking of the B cell receptor Ig-alpha/beta subunit in a B lymphoma cell line. Journal of Immunol. 165: 1427-1437
  14. Gold, M.R., Scheid, M.P., Santos, L., Matsuuchi, L., Duronio, V. and Krebs, D.L. 1999. Regulation of the AKT/PKB/Glycogen synthase kinase-3 signaling pathway by the B cell antigen receptor. Journal of Immunol. 163:1894-1905
  15. Richards, J.D., Gold, M.R., Hourihane, S.L., DeFranco, A.L. and Matsuuchi, L. 1996. Reconstitution of B cell antigen receptor-induced signaling events in a nonlymphoid cell line by expressing the syk protein tyrosine kinase. Journal of Biological Chemistry 271:6458-6466
Office Phone
6048224881
Office
3457