Research Summary

The lab investigates excitation-contraction coupling in muscle cells. We utilise high resolution three-dimensional fluorescence microscopy as well as transmission and 3-D electron microscopy to understand structure-function relationships in cells. We are particularly interested in the role of caveolae in cardiac muscle, and employ a combination of 3D imaging, contraction studies, and viral gene transfection to investigate normal and abnormal hearts. In addition to the cardiovascular system, we have collaborative projects with other laboratories interested in gastroenterology, endocrinology, neurophysiology, ion channels and lipid metabolism.

Technologies & Methods
Bio

B.Sc. and M.Sc. Physiology, University of Toronto
Ph.D. Pharmacology, Mayo Clinic

Publications

 
Comprehensive List
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Selected Publications

  1. Scriven DRL, Asghari P and Moore EDW.
    Microarchitecture of the dyad.
    Cardiovasc. Res. 2013 May 1;98(2):169-76. Epub 2013 Feb 11.
  2. Scriven DRL and Moore EDW.
    Ca(2+) channel and Na(+)/Ca(2+) exchange localization in cardiac myocytes.
    J. Mol. Cell. Cardiol. 2013 May; 58:22-31. Epub 2012 Dec 7.
  3. Bround MJ, Asghari P, Wambolt R, Bohunek L, Smits C, Philit M, Kieffer TJ, Lakatta EG, Boheler KR, Moore EDW, Allard MF, Johnson JD
    Cardiac ryanodine receptors control heart rate and rhythmicity in adult mice.
    Cardiovasc. Res. 2012 Dec 1; 96(3) : 372-80.
  4. Asghari P, Scriven DRL, Hoskins J, Famelli N, van Breeman C, and Moore EDW
    The structure and functioning of the couplons in the mammalian cardiomyocyte
    Protoplasma 2012; 249(Suppl 1): S31-S38.
  5. Schulson MN, Scriven DRL, Fletcher P, and Moore EDW
    Couplons in rat atria form distinct subgroups defined by their molecular partners
    J. Cell Sci. 2011 Mar 8; 124(7): 1167-1174.
  6. Scriven DRL, Asghari P, Schulson MN and Moore EDW
    Analysis of Cav1.2 and Ryanodine Receptor Clusters in Rat Ventricular Myocytes
    Biophys. J. 2010 Dec 15; 99(12): 3923-3929.
  7. Fletcher PA, Scriven DRL, Schulson MN and Moore EDW
    Multi-image Colocalization and its Statistical Significance
    Biophys. J. 2010 Sep 22; 99(6): 1996-2005.
  8. Perkins AD, Ellis SJ, Asghari P, Shamsian A, Moore EDW and Tanentzapf G
    Integrin-mediated adhesion maintains sarcomeric integrity
    Dev. Biol. 2010 Feb 1; 338(1): 15-27
  9. Asghari P, Schulson M, Scriven DRL, Martens G and Moore EDW
    Axial Tubules of Rat Ventricular Myocytes Form Multiple Junctions with the Sarcoplasmic Reticulum.
    Biophysical J. 2009 June 3; 96(11): 4651-4660.
  10. Chopra N, Yang T, Asghari P, Moore ED, Huke S, Akin B, Cattolica RA, Perez CF, Hlaing T, Knollmann-Ritschel BEC, Jones LR, Pessah IN, Allen PD, Franzini-Armstrong C and Knollmann BC.
    Ablation of triadin causes loss of cardiac Ca2+ release units, impaired excitation-contraction coupling, and cardiac arrhythmias
    PNAS 2009 May 5; 106: 7636-7641.
  11. Scriven DRL, Lynch RM and Moore EDM.
    Image Acquisition for Colocalization Using Optical Microscopy.
    Am J Physiol Cell Physiol. 2008 May; 294(5): C1119-22. Epub 2008 Mar 19.
  12. Lynch RM, Weber CS, Nullmeyer KD, Moore ED and Paul RJ.
    Clearance of store-released Ca2+ by the Na+-Ca2+ exchanger is diminished in aortic smooth muscle from Na+-K+-ATPase {alpha}2-isoform gene-ablated mice.
    Am J Physiol Heart Circ Physiol. 2008 Mar;294(3):H1407-16. Epub 2008 Jan 11.
  13. Gaudreault N, Scriven DRL, Laher I and Moore EDW.
    Subcellular characterization of glucose uptake in coronary endothelial cells.
    Microvascular Research 2008 Jan;75(1):73-82. Epub 2007 Apr 25.
  14. Eldstrom J, Van Wagoner DR, Moore ED and Fedida D
    Localization of Kv1.5 channels in rat and canine myocyte sarcolemma.
    FEBS Lett. 2006 Nov 13; 580(26):6039-46.
  15. Gaudreault N, Scriven DRL and Moore EDW
    Asymmetric subcellular distribution of glucose transporters in the endothelium of small contractile arteries.
    Endothelium. 2006 Sep-Oct; 13(5):317-24.
  16. Scriven DRL, Klimek A, Asghari P, Bellve K and Moore EDW
    Caveolin-3 Is Adjacent To A Group Of Extra-Dyadic Ryanodine Receptors.
    Biophys J. 2005 Sep; 89(3) :1893-1901.
  17. Guzzo RM, Salih M, Moore ED and Tuana BS
    Molecular properties of cardiac tail-anchored membrane protein SLMAP are consistent with structural role in arrangement of excitation-contraction coupling apparatus.
    Am J Physiol Heart Circ Physiol. 2005 Apr;288(4):H1810-9.
  18. Gaudreault N, Scriven DRL and Moore EDW
    Characterisation of glucose transporters in the intact coronary artery endothelium in rats: GLUT-2 upregulated by long-term hyperglycaemia.
    Diabetologia. 2004 Dec;47(12):2081-92.
  19. Moore ED, Voigt T, Kobayashi YM, Isenberg G, Fay FS, Gallitelli MF, Franzini-Armstrong C.
    Organization of Ca2+ release units in excitable smooth muscle of the Guinea-pig urinary bladder.
    Biophys J. 2004 Sep;87(3):1836-47.
  20. Conlin VS, Curtis SB, Zhao Y, Moore ED, Smith VC, Meloche RM, Finlay BB, Buchan AM
    Helicobacter pylori infection targets adherens junction regulatory proteins and results in increased rates of migration in human gastric epithelial cells.
    Infect Immun. 2004 Sep;72(9):5181-92.
  21. Sedarat F, Lin E, Moore ED and Tibbits GF
    Deconvolution of confocal images of Dihydropyridine and Ryanodine Receptors in developing cardiomyocytes
    J Appl Physiol. 2004 Sep;97(3):1098-103.
  22. Guzzo RM, Wigle JT, Salih M, Moore ED and Tuana BS
    Regulated expression and temporal induction of the tail-anchored membrane protein SLMAP is critical for myoblast fusion.
    Biochem J. 2004 Aug;381(Pt 3):599-608.
  23. Wang Z, Eldstrom JR, Jantzi J, Moore ED and Fedida D.
    Increased focal Kv4.2 channel expression at the plasma membrane is the result of actin depolymerization
    Am J Physiol Heart Circ Physiol. 2004 Feb;286(2):H749-59.
  24. Dan P, Cheung JC, Scriven DRL and Moore ED
    Epitope Dependent Localization of Estrogen Receptor alpha, but not beta, in En Face Arterial Endothelium
    Am J Physiol Heart Circ Physiol. 2003 Apr;284(4):H1295-306.
  25. Lagaud G, Gaudreault N, Moore ED, Van Breemen C and Laher I.
    Pressure-dependent myogenic constriction of cerebral arteries occurs independently of voltage-dependent activation.
    Am J Physiol Heart Circ Physiol. 2002; 283(6):H2187-95.
  26. Scriven DRL, Klimek A, Lee K and Moore ED
    The Molecular Architecture of Calcium Microdomains in Rat Cardiomyocytes
    Ann NY Acad Sci 2002; 976:488-499.
  27. Scriven DR, Dan P, Moore ED.
    Distribution of proteins implicated in excitation-contraction coupling in rat ventricular myocytes.
    Biophys J. 2000; 79(5):2682-91.
  28. Sedarat F, Xu L, Moore ED, Tibbits GF.
    Colocalization of dihydropyridine and ryanodine receptors in neonate rabbit heart using confocal microscopy.
    Am J Physiol Heart Circ Physiol. 2000; 279(1):H202-9.
  29. Maruoka ND, Steele DF, Au BP, Dan P, Zhang X, Moore ED, Fedida D.
    alpha-actinin-2 couples to cardiac Kv1.5 channels, regulating current density and channel localization in HEK cells.
    FEBS Lett. 2000; 473(2):188-94.
  30. Lougheed M, Moore ED, Scriven DR, Steinbrecher UP.
    Uptake of oxidized LDL by macrophages differs from that of acetyl LDL and leads to expansion of an acidic endolysosomal compartment.
    Arterioscler Thromb Vasc Biol. 1999; 19(8):1881-90.
  31. Moore ED, Ring M, Scriven DR, Smith VC, Meloche RM, Buchan AM.
    The role of protein kinase C isozymes in bombesin-stimulated gastrin release from human antral gastrin cells.
    J Biol Chem. 1999; 274(32):22493-501.
  32. Meininger GA, Moore ED, Schmidt DJ, Lifshitz LM, Fay FS.
    Distribution of active protein kinase C in smooth muscle.
    Biophys J. 1999; 77(2):973-84.
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