Tissue Processing

Tissue Processing

The Tissue Processing Facility is suitable for performing immunohistochemical and immunofluorescence analysis of tissue slices (e.g. lymphoid organs, pancreas). The facility has a high quality semi-electronic cryostat as well as an Olympus BX61 motorized fluorescence microscope.

Before Booking…
Please take the time to read over the following policies.

Training (PDF)
Online Calendar (PDF)
Fees (PDF)

Data Transfer (PDF)
Access (PDF)


 
 

Olympus BX61 Motorized Fluorescence Microscope

The BX61 is a fully motorized research microscope built on the platform of the traditional upright frame. All major microscope functions can be completely automated, including focus, illumination, objective lens selection and filter wheels. A complete software command set allows for full computer control.

 TP_olympus-microscope
 

$5/h for LSI Members
& non-LSI Members

Features:

  • Olympus DP72 12.8 MP cooled
  • Colour CCD camera
  • Olympus cellSens Dimension imaging
  • Software, including 5D acquisition and Automated Full Count & Measure modules.
  • Super Apochromatic objective lenses – 4x, 10x, 20x, 40x, 60x oil, 100x oil Internal motorized Z-drive for automated Z-stacks and
  • Extended Focus Imaging (EFI)
  • 6 position motorized fluorescence
  • Turret for automated multichannel fluorescence acquisition
  • 6 position motorized nose-piece
  • 8 position manual condenser
  • Right hand manual XY stage
  • Trinocular observation tube
  • Differential Interference Contrast
  • (DIC) optics
  • Exfo x-Cite metal halide fluorescence
  • Light source with integrated iris
  • Semrock hard-coated Zero Pixel
  • Filter cubes – DAPI, FITC, TRITC, Texas Red Imaging computer workstation with high performance wide-screen 24″ LCD

Thermo Scientific Microm HM 525 Cryostat

The LSI Thermo Scientific HM 525 Cryostat is a semi-electronic cryostat with stepper motor advance that provides superior consistency and reliability of sectioning results.

 TP_cryostat
 

$5/h for LSI Members
& non-LSI Members

Features:

  • Open top cryostat with stainless steel cooling chamber.
  • Variable temperature regulation with automatic defrost cycle and integrated fast freezing device down to max -60°C.
  • Sliding window with heater and fluorescent lamp for cryo-chamber.
  • Motorized coarse feed in two directions with limit indication and automated switch off at front and rear limits of horizontal travel.
  • Section counter and indication of sum of section thickness.
  • Chamber temperature control:+5°C to -35°C
  • Fast freezing station: max -60°C

Microtome Features:

  • Section thickness: 1 to 500µm
  • Fine Section thickness: 1 to 20µm
  • Trimming thickness: 10 to 500 µm
  • Specimen retraction during return travel: 40 µm
  • Vertical specimen stroke: 60 mm
  • Horizontal specimen movement: 28 mm

Specimen Orientation:

  • x- and y- axes: universal 8° with zero positioning
  • z-axis: rotatable 360°


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