LSI TEM Facility

LSI TEM Facility

The LSI Transmission Electron Microscopy (TEM) facility is the home to the TEM and ultramicrotome purchased from the CFI grant received by Drs. Robert Molday, Wayne Vogl, Nelly Pante and Calvin Yip.  The TEM has a high level of automation and is suitable for applications like 3D-imaging using tomography and/or cryo-electron microscopy.

Before Booking…

Please take the time to read over the following TEM policies.

General (PDF)
Training (PDF)
Online Calendar (PDF)

Fees (PDF)
Data Transfer (PDF)
Access (PDF)

FEI Tecnai G2 Spirit Transmission Electron Microscope

The LSI FEI Tecnai G2 Spirit ensures high-quality images, is easy to use and is designed to facilitate the work of the microscope user. It has a high level of automation, yet doesn’t limit full control for experienced, expert users. The microscope is ready for novel applications like 3D-imaging using tomography and/or cryo-electron microscopy.

TEM_fei-tecnai-spirit

Features:

  • High contrast, high resolution for 20 –120 kV operation.
  • Optimized for 2D & 3D imaging of cells, cell organelles and soft matter.
  • High level of automation: Auto-Gun and automatic tuning.
  • Smart Tracking Position System for sample navigation.
  • Customized protocols for diverse applications.
  • Sample observation at liquid nitrogen temperature.
  • Technology for sharp imaging of thicker samples.
  • Ergonomic design for operational comfort.

$40/h for LSI Members
$60/h for non-LSI Members

Leica Ultramicrotome

Ultramicrotome for ultra-thin sectioning of biological and industrial samples.

TEM_microtome

Features:

  • Up to 100 different user/specimen/knife profiles can be set.
  • Electronic data transfer for reporting user, specimen, knife and storage parameters.
  • Knife usage monitoring.
  • Optimized ionizer.
  • Fully motorized knife stage.
  • AutoTrim function.
  • Brightness-controlled.
  • Multi-LED illumination.
  • LED spot illumination.

free for LSI Members
$20/h for non-LSI Members

Richert-Jung Ultracut E Microtome

Ultramicrotome for ultra-thin sectioning of biological and industrial samples.

TEM_ultracut

Features:

  • Micro specimen thickness range 0 to 0.5 microns.
  • Ultra specimen thickness range 0 to 0.14 microns.
  • Motorized cutting stroke with adjustable speed 0.1 to 90 mm/sec.
  • Sectioning window adjustable 0.5 to 22mm.
  • Manual sectioning possible with built in handwheel.
  • AO Stereo Star microscope with zoom factor 0.7 to 4.2X and 10X eyepieces

free for LSI Members
$20/h for non-LSI Members


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