Research Summary

Our laboratory has a dynamic team of individuals focused on understanding the causes of type 1 and type 2 diabetes at a molecular level. Our studies are guided by the discovery of genes and associated gene networks linked to diabetes risk and by known risk factors that predispose individuals to diabetes. The common forms of both type 1 diabetes and type 2 diabetes appear to result from a combination of genetic and acquired factors, and both diseases are increasing in prevalence. Despite some major advances, we do not yet understand the root causes of diabetes.

We study the role of the insulin-secreting pancreatic beta-cell in type 1 diabetes, type 2 diabetes, and other rare forms of diabetes. We are particularly interested in determining the molecular signalling pathways that control the survival and function of these insulin-secreting cells. These signals represent the key to understanding the disease and designing rational treatments.

In order to understand these processes, we employ state-of-the-art techniques including: molecular imaging, molecular biology and in vivo studies. In many cases we examine the role of a particular gene from the single-cell level (where the exact mechanism of its action can be established) to the level of the whole organism (where its role in total body energy homeostasis can be evaluated).

In our Laboratory, we believe fundamental science is essential to finding a cure to diabetes. The lab is filled with motivated, hard-working staff, students and post-doctoral fellows working toward this goal.

Technical Expertise

  • Optical single cell recording techniques, including multiple wavelength dye and fluorescent protein imaging (e.g. Fura calcium imaging, GFP-tagging and localization).
  • Forster resonance energy transfer imaging (FRET), frequency domain fluorescence lifetime imaging microscopy (FLIM; FLIM-FRET).
  • Total internal reflectance fluorescence imaging microscopy (TIRF).
  • Advanced data analysis, techniques for image quantification and manipulation.
  • 3D imaging and cell volume analysis in cardiomyocytes.
  • High-content, high-throughput imaging.
  • Proteomics (fluorescent 2D-DIGE), genomic analysis
  • Primary culture of endocrine cells for physiological measurements of hormone secretion, production, gene expression, radioimmunoassay and ELISA.
  • Immunohistochemistry, cell proliferation, apoptosis.
  • Morphological identification of live cells.
  • Single cell microinjection.
  • Basic molecular biology, including shRNAi, gene manipulation.
  • Basic cellular biochemistry, DNA ladders, Western blot.
  • In vivo and in vitro phenotype analysis of transgenic and mutant mice.
  • Projects

    Causal Roles of Hyperinsulinemia in Obesity and Altered Longevity
    The epidemics of obesity, type 2 diabetes and related diseases threaten to overrun the global healthcare system. We know that obesity, insulin resistance, early type 2 diabetes are all highly correlated with each other and are all associated with a higher than normal release of insulin from the pancreas, but we still do not fully understand the causal relationship between these phenomena. The most commonly accepted view is that obesity first leads to insulin resistance, which then leads to a compensatory hypersecretion of insulin, which finally results in diabetes when insulin release from the pancreas fail to meet demands. However, these implied cause and effect relationships have been questioned and are impossible to formally test in humans using rigorous genetic loss-of-function approaches. Indeed, there has long been evidence that basal insulin hypersecretion can precede insulin resistance and even obesity and clinical studies have also pointed to anti-obesity effects of drugs that block insulin secretion. We are testing the hypothesis that pancreatic insulin causes obesity directly, by genetically eliminating half of the insulin gene from the pancreas. We expect our study to have an important impact on our fundamental understanding of obesity, which might change the way we diagnose and treat millions of people. This work is funded by the Canadian Institutes of Health Research.

    Roles of Brain-Produced Insulin in Alzheimer’s Disease
    Many previous reports have suggested that small amounts of insulin may be produced locally by adult central neurons of mammals, including humans. Johnson’s group has recently confirmed these reports using rigorous negative and positive controls, but the function of centrally produced insulin remains a mystery. In this project, we will selectively delete the insulin 2 gene from the mouse brain in order to determine the role of central insulin. Previous studies have suggested that brain insulin is reduced in Alzheimer’s disease, making these studies highly relevant to human disease. This work is funded by the Alzheimer’s Society of Canada.

    Hyperinsulinemia and Insulin Signalling in Pancreatic Cancer
    Pancreatic adenocarcinoma is the fourth most common cause of cancer death in Canada, but receives the lowest proportion of research funding of any major cancer. As a result, our understanding of the factors that initiate and drive the progression of this disease remains poor relative to our knowledge pertaining to other cancers. Diabetes mellitus and obesity are emerging as important risk factors for pancreatic cancer and the rapid rise in BMI foreshadows a rise in pancreatic cancer. Elevated insulin levels are a feature of both obesity and type 2 diabetes. Hyperinsulinemia has been investigated as a possible contributor to cancer initiation and progression. Lowering hyperinsulinemia with metformin was shown to reduce the risk of pancreatic cancer by 60%. Groups in Europe created headlines worldwide by showing an increased risk of cancer with use of long-acting insulin analogues. The question of whether elevated insulin can be a causal in the pathogenesis of pancreatic cancer has not been rigorously tested. To test this hypothesis, we have established models engineered to lack multiple alleles of their two Insulin genes. Mice with two of four Insulin alleles are hyperinsulinemic on a high-fat diet, whereas mice lacking all but one Insulin allele are hypoinsulinemic but not diabetic. We also have mouse models that will allow us to test whether genes involved in insulin-stimulated proliferation and anti-apoptosis in pancreatic islets, Raf-1, Akt or Pdx-1, participate in pancreatic cancer. Whether insulin signalling might synergize with Kras, the most frequently mutated gene in pancreatic cancer, is a key unanswered question. The overall goal is to test the hypothesis that hyperinsulinemia in diabetes can contribute to hyperproliferation in the exocrine pancreas, promote pre-cancerous lesions, and promote the survival of pancreatic cancer cells. We will also test the hypothesis that Raf-1, Akt and Pdx-1 are critical for insulin action in the pancreas. Together, these studies have the potential to increase our understanding of this devastating disease and increase avenues towards rational therapeutic intervention. This information will eventually be used to identify novel compounds capable of blocking the hyperproliferative and anti-apoptotic effects of insulin in primary human pancreatic tissue and pancreatic tumor cell lines. This work has been funded by the Cancer Research Society of Canada.

    Insulin Receptor Trafficking and Insulin ‘Feedback’ Signalling in Type 1 and Type 2 Diabetes
    Insulin is both a metabolic hormone and growth factor. The signal transduction cascades activated by insulin have been well studied in ‘insulin target tissues’ such as muscle and fat. However, many studies have revealed unexpected tissues where blocking insulin signalling has adverse consequences to glucose homeostasis. Surprisingly, along with the liver and brain, these studies show that the pancreatic beta-cell itself an important site of insulin action. In addition, islets from human type 2 diabetics appear to be ‘insulin resistant’. We have investigated the role and mechanism of insulin signalling in the beta-cell and we have uncovered exciting differences compared to other tissues. We are continuing to study the effects of insulin on primary human and mouse islets, focusing on the anti-apoptotic effects of insulin and the mechanism of these effects. We have focused on signalling pathways regulated by the Raf-1 kinase and related proteins. We are testing the hypothesis that altered insulin expression plays a role in type 1 diabetes. Recently, we have developed a new way of following the movements of functional insulin receptors in living cells, and used this technology to make insights into insulin signal transduction. This project has been supported by grants from the Juvenile Diabetes Research Foundation and the Canadian Institute for Health Research.

    High-Content Screening for Molecules that Promote Beta-Cell Survival
    Virtually any cure for type 1 diabetes will require strategies to protect beta-cells from death and dysfunction. With recent support from JDRF, they have optimized novel imaging technologies that allow for the first time the simultaneous, real-time analysis beta-cell function and programmed cell death, on a single-cell basis. At the same time, using bioinformatics and genomics, our laboratory has compiled and published a list of 234 locally produced secreted factors and 233 secreted factor receptors. High-throughput screening approaches now make it possible to examine simultaneously all of these potential survival and differentiation factors under multiple conditions related to the pathogenesis of type 1 diabetes. The overall goal of the proposed study is to identify the most powerful locally acting survival and/or differentiation factors in human islets. Such a factor could be harnessed to improve graft survival in clinical islet transplantation, improve the production surrogate beta-cells, and eventually treat patients with type 1 diabetes and/or their at-risk family members. This work has been funded by the JDRF.

    High-Content Screening for Molecules that Regulate Beta-Cell Differentiation Status
    A major goal of regenerative medicine is the generation of fully functional pancreatic beta-cells, either from residual beta-cells in the patient or from stem cells. Both of these therapeutic avenues require a thorough understanding of the process by which beta-cells go from being immature to fully functional beta-cells. With SCN support, we devised a method to examine the maturation of single beta-cells for the first time using powerful custom microscopes and state-of-the-art fluorescent markers. At the same time, we have built (using CFI funds) the infrastructure to perform these experiments in a massively parallel manner. In doing so, we have become one of Canada’s leading centres for image-based screening in the diabetes field. This work has been funded by the Stem Cell Network and the JDRF.

    New Roles for RyR2-Mediated Calcium Flux in Cardiomyocyte Survival, Metabolism
    Every heartbeat is composed of a complex cycle of highly orchestrated events. The cardiac ryanodine receptor calcium channel (RyR2) is central to this cycle, releasing calcium to cause heart muscle cell contraction with each heartbeat. Diseases such as arrhythmias and diabetic cardiomyopathy are associated with changes in RyR2 function. However, it has remained unclear whether the other cellular symptoms of these conditions are causes or consequences of the loss of RyR2 function. Working in other cell types, we have recently described unexpected roles for RyR2, namely the control of gene expression and cell survival. In this project we examine hearts with selective deletion of RyR2 calcium channels and determine which cellular functions are changed most directly as a result. These studies will provide new insight into the dysfunction and death of heart cells in disease.

    Calcium-Dependent Signal Transduction in Pancreatic Beta-Cells
    All cellular processes are controlled by signals. Defects in the transduction of these signals cause disease. Although we have learned a great deal about the events that control a variety of functions in pancreatic beta-cells, the signalling defects that cause diabetes remain to be elucidated. A major interest in the laboratory is the role of intracellular calcium stores, including those sensitive to IP3, ryanodine and NAADP, in beta-cell survival and function. Intracellular calcium homeostasis is vital to the survival of all cell types. We are particularly interested in the mechanisms by which dysfunctional intracellular calcium signalling leads to programmed cell death. Intracellular calcium stores have been linked to diabetes in previous studies. There is also strong evidence that ER-stress, resulting from lowered ER calcium levels in the beta-cell, plays a significant role in both rare and common forms of diabetes. We are currently using advanced biochemical and molecular techniques, including FRET-based imaging for calcium signals, to further this research.

    Lipotoxicity and Gene-Environment Interactions in Type 2 Diabetes
    One of the causes of type 2 diabetes is an increase in pancreatic beta-cell death, leading to insufficient insulin. Unfortunately, we still do not understand which genes are required for beta cell survival in the presence of high fat, so we are unable to design effective treatments to stop beta-cell death. For decades, medical research has primarily used a ‘candidate’ gene approach to study known proteins, one at a time, for their role in specific disease states. With the emergence of new technology and systems biology, it is now possible to simultaneously examine virtually all genes or proteins in a cell. These unbiased approaches reveal novel findings that could not have been predicted based on prior knowledge. We have used unbiased proteomic analysis to reveal part of the mechanism by which fatty acids kill beta-cells via ER-stress. The objective of this research program is to continue our proteomics-guided efforts to understand how fatty acids kill beta cells. In particular, we will focus how fatty acids alter the beta-cell’s quality control system for proteins such insulin. We use advanced molecular biology and microscopy to determine how fatty acids lead to the degradation of key proteins in beta-cells. This study will improve our understanding of the underlying causes of diabetes as we search for ways to prevent, manage and cure this disease. This project is supported by a grant-in-aid from the Canadian Diabetes Association.

    Bio

    PhD Cell Biology and Physiology, University of Alberta
    Post-Doctoral Fellowship, Washington University Medical Center

    Awards

    2014 Killam Research Fellowship
    2009 Researcher of the Year – Department of Cellular and Physiological Sciences
    2008 UBC Faculty of Medicine Distinguished Achievement Award for Excellence in Basic Science Research
    2007 Murray L Barr Award, Canadian Association for Anatomy Neurobiology and Cell Biology
    2006 Canadian Diabetes Association Scholarship (declined)
    2006 Canadian Institute of Health Research New Principal Investigator Award
    2005 Juvenile Diabetes Research Foundation Career Development Award

    Publications

    Corresponding author = †. A brief description of my role in the paper accompanies each publication. My unique ISI Researcher ID is B-3919-2008 (see www.researcherid.com). My citation metrics are public on Google Scholar Citations.

    1. Johnson JD†, Bernal-Mizrachi E, Alejandro E, Han Z, Kalynyak T, Li H, Beith JL, Gross J, Warnock GL, Townsend RR, Permutt MA, Polonsky KS. 2006. Insulin protects islets from apoptosis via pdx-1 and specific changes in the human islet proteome. Proc. Natl. Acad. Sci. USA. (Track II).103: 19575-80.
      •• Provides evidence for anti-apoptotic effects of insulin on mouse and human beta-cells. 1st proteomic analysis of the human islet proteome under 2 conditions.
    2. Brunham LR, Kruit JK, Pape TD, Timmins JM, Reuwer AQ, Vasanji Z, Marsh BJ, Johnson JD, Parks JS, Verchere CB, Hayden MR. 2007. B-Cell ABCA1 Influences Insulin Secretion, Glucose Homeostasis and Response to Thiazolidinedione Treatment. Nature Medicine. 13: 230-7.
      •• 1st study on the role of any cholesterol transporter in beta-cells.
    3. Dror V, Nguyen V, Walia P, Kalynyak TB, Hill JA, Johnson JD†. 2007. Notch signalling suppresses apoptosis in adult pancreatic islet cells. Diabetologia 50:2504-15.
      •• 1st study of Notch signalling in adult islets. 1st demonstration that Neurogenin 3 protein is present and can be modulated in adult islets (the presence of Neurogenin 3 was controversial at the time but has since been vindicated).
    4. Beith JL, Alejandro EU, Johnson JD†. 2008. Insulin stimulates primary β-cell proliferation via raf-1 kinase. Endocrinology. 149:2251-60.
      •• 1st direct demonstration that insulin, but not glucose, stimulates proliferation in single primary beta-cells.
    5. Johnson JD†, Alejandro EU. 2008. Control of Pancreatic Beta-cell Fate by Insulin Signaling: The Sweet Spot Hypothesis. Cell Cycle. Invited review. EPub. March 3, 2008. May 15;7(10):1343-7.
      •• Proposed several new concepts in the field.
    6. Jeffrey KD, Alejandro EU, Luciani DS, Kalynyak TB, Hu X, Li H, Lin Y, Townsend RR, Polonsky KS, Johnson JD†. 2008. Carboxypeptidase E mediates palmitate-induced beta-cell ER-stress and apoptosis. Proc. Natl. Acad. Sci. USA. (Track II). Jun 17;105(24):8452-7.
      •• 1st use of proteomics to identify pathways that mediate lipotoxity in human islets. Discovered novel mechanistic links between free fatty acids, insulin processing via carboxypeptidase E and type 2 diabetes. This article attracted significant local and national media attention. Recommended in F1000Prime.
    7. Szabat, M, Johnson, JD†, Piret JM. 2010. Reciprocal modulation of adult beta-cell maturity by activin A and follistatin. Diabetologia. 53: 1680-9.
      •• 1st identification of soluble factors that modulate beta-cell maturity state. Insight came from a small factorial screen.
    8. Yang YHC, Szabat M, Bragagnini C, Kott K, Helgason CD, Hoffman BG, Johnson JD†. 2011. Paracrine signaling loops in adult pancreatic islets: Netrins modulate beta-cell apoptosis via Neogenin and Unc5a. Diabetologia 54(4):828-42.
      •• 1st published bioinformatic list of secreted peptide factors from islets and receptors for those factors in islets. 1st study of the role of netrins in adult islets.
    9. Alejandro EU, Lim GE, Mehran AE, Hu X, Taghizadeh F, Pelipeychenko D, Baccarini M, Johnson JD†. 2011. Pancreatic beta-cell Raf-1 is required for glucose tolerance, insulin secretion and insulin 2 transcription. FASEB J 25(11):3884-95.
      •• 1st analysis of any Raf isoform in beta-cells. 1st evidence that reducing insulin production is sufficient to impair insulin secretion and glucose homeostasis (insulin production was previously not thought to be rate-limiting).
    10. Chu KY, Li H, Wada K, Johnson JD†. 2011. Ubiquitin C-terminal hydrolase L1 is required for β-cell survival and function in lipotoxicity. Diabetologia. 285(42):32606-15.
      •• 1st demonstration that Uchl1, known for its role in Parkinson’s disease, is required for beta-cell survival and function in vivo.
    11. Luciani DS, White SA, Widenmaier S, Saran V, Taghizadeh F, Hu X, Allard MF, Johnson JD†. 2012. Bcl-2 and Bcl-xL suppress glucose signalling in pancreatic beta-cells. Diabetes 62:170-82.
      •• 1st demonstration that ‘pro-survival’ Bcl family members regulate glucose sensing at the level of mitochondrial metabolism in beta-cells and glucose homeostasis in vivo.
    12. Bround MJ, Asghari P, Wambolt R, Bohunek L, Smits C, Philit M, Kieffer TJ, Lakatta EG, Boheler KR, Moore EDW, Allard MF, Johnson JD†. Cardiac ryanodine receptors control heart rate and rhythmicity in adult mice. Cardiovascular Research. EPub August 6, 2012.
      •• 1st demonstration that Ryr2 channels maintain heart rate and prevent arrhythmias in vivo, which was a source of great controversy.
    13. Mehran AE, Templeman NM, Brigidi GS, Lim GE, Chu K-Y, Hu X, Botezelli JD, Asadi A, Hoffman BG, Kieffer TJ, Bamji SX, Clee SM, Johnson JD†. 2012. Hyperinsulinemia drives diet-induced obesity independently of brain insulin production. Cell Metabolism. 16, 723–737.
      •• 1st demonstration that hyperinsulinemia is required for diet-induced obesity, a concept that was a source of speculation and controversy for several decades; 1st demonstration that any established hormone can reprogram white fat to exhibit energy burning qualities of brown fat; 1st demonstration that insulin is produced locally in the brain that included both positive and negative controls; 1st demonstration that insulin controls beta-cell mass expansion.
    14. Lim GE, Piske M, Johnson JD†. 2013. 14-3-3 proteins are essential signalling hubs for beta cell survival. Diabetologia. EPub Jan 25.
      •• 1st demonstration of a role for 14-3-3 proteins in beta-cells; 1st side-by-side comparison of 14-3-3 proteins in any cell type.
    15. Wang M, Li J, Lim GE, Johnson JD†. 2013. Is dynamic autocrine insulin signaling possible? A mathematical model predicts picomolar concentrations of extracellular monomeric insulin within human pancreatic islets. PLOS ONE April 19, 2013.
      •• 1st mathematical model to estimate the levels of monomeric insulin within pancreatic islets; lends plausibility to the concept that insulin is a physiologically relevant modulator of beta-cells in vivo.
    16. Bround MJ, Wambolt R, Luciani DS, Kulpa JE, Rodrigues B, Brownsey RW, Moore EDW, Allard MF, Johnson JD†. 2013. Cardiomyocyte ATP production, metabolic flexibility, and survival require calcium flux through cardiac ryanodine receptors in vivo. JBC. EPub May 15, 2013.
      •• 1st in vivo demonstration that calcium release from intracellular stores regulates mitochondrial metabolism, metabolic gene expression, and survival in any cell type. Shows that RYR2 is the key link between cardiomyocyte cell function, metabolism and fate.
    17. Yang YHC, Manning-Fox JE, Zhang KL, MacDonald PE, Johnson JD†. 2013. Autocrine SLIT-ROBO signaling is required for beta-cell survival and potentiates insulin secretion. PNAS. Epub Sept 24, 2013.
      •• 1st analysis of SLIT-ROBO signalling in pancreatic islet cells. One of the 1st examples of a locally produced growth factor, other than insulin, that is required for beta-cell survival.
    18. Yang YHC, Vilin YY, Roberge M, Kurata HT, Johnson JD†. Multi-parameter screening reveals a role for Na+ channels in cytokine-induced beta-cell death. Molecular Endocrinology EPub Jan 21, 2014.
      •• First demonstration of a role for Na+ channels in beta-cell death. Identification of a novel small molecule that blocks apoptosis in response to type 1 diabetes associated stresses.
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