Dr. Moerman is currently part of an international consortium dedicated to identifying mutations in all 20,000 genes of the free-living nematode C. elegans (see for example, http://genome.sfu.ca/mmp/search.html) . In the past he has utilized this model system to address fundamental questions in the process of muscle assembly. Another current effort in his laboratory focuses on using C. elegans as a surrogate for parasitic nematodes to discover new anthelmintics. Using a new high throughput screening method he and his group are identifying a range of new chemistries that affect nematode growth, development and fecundity.
Donald Moerman is a Professor in the Department of Zoology at the University of British Columbia. He received his BSc and PhD degrees in Biology from Simon Fraser University and then held a MRC Postdoctoral Fellowship in Genetics at Washington University School of Medicine. Prior to joining UBC as an Assistant Professor in 1987, Dr. Moerman was a Research Associate and Research Assistant Professor at Washington University (1983-87). From 1994-95, Dr. Moerman was a Killam Faculty Research Fellow and a Visiting Scientist at the Max-Planck Institute for Biochemistry in Martinsried, Germany.
Gene Knockout Lab Publications
1. Edgley, M., A. D’Souza, G. Moulder, S. McKay, B. Shen, E. Gilchrist, D. Moerman and R. Barstead. 2002 Improved detection of small deletions in complex pools of DNA. Nucleic Acids Research 30: e52.
2. Barstead R.J. and Moerman D.G. 2006 C. elegans deletion mutant screening. Methods Mol. Biol. 2006;351:51-8.
3. Maydan, J.S., Flibotte, S., Edgley, M.L., Lau, J., Selzer, R.R., Richmond, T.A., Pofahl, NJ., Thomas, J.H., Moerman, D. 2007 Efficient high-resolution deletion discovery in Caenorhabditis elegans using oligonucleotide by Array Comparative Genomic Hybridization. Genome Research 2007 Mar;17(3):337-47
4. Moerman, D. G., and R. J. Barstead. 2008 Towards a mutation in every gene in Caenorhabditis elegans. Brief. Funct. Genomic Proteomic 7: 195–204.
5. Maydan, J.S., H.M. Okada, S. Flibotte, M.L. Edgley and D.G. Moerman. 2009. De novo identification of single nucleotide mutations in C. elegans using array Comparative Genomic Hybridization. Genetics 181: 1673-1677.
6. Flibotte, S., M. Edgley, J. Maydan, J. Taylor, R. Zapf, R. Waterston, and D.G. Moerman. 2009. Rapid High Resolution SNP-CGH mapping in Caenorhabditis elegans. Genetics 181(1):33-7.
Muscle Research Group Publications
1. Rogalski, T., B. Williams, G. Mullen and D.G. Moerman. 1993. Products of the unc-52 gene in Caenorhabditis elegans are homologous to the core protein of the mammalian basement membrane heparan sulfate proteoglycan. Genes & Development 7: 1471-1484.
2. Moerman, D.G. and A. Fire. 1997. Muscle: structure, function and development. pp417-470. In Nematodes II, edited by D.L. Riddle, T. Blumenthal, B.J. Meyer, and J.R. Priess, Cold Spring Harbor Laboratory Press. N.Y.
3. Hobert, O., D.G. Moerman, K.A. Clark, M.C. Beckerle and G. Ruvkin 1999 A conserved LIM protein that affects muscular adherens junction integrity and mechanosaensory function in Caenorhabditis elegans. Journal of Cell Biology 144:45-57. [A picture from our paper graces the cover of this issue of the journal].
4. Rogalski, T.M., G.M. Mullen, M. Gilbert, B. Williams and D.G. Moerman. 2000 UNC-112: a new protein required for myofilament initiation and organization in C. elegans . J. Cell Biol. 150: 253-264. [This paper was the subject of a review in JCB]
5. Norman, K. and D.G. Moerman. 2000 The let-268 locus of C. elegans encodes a lysyl hydroxylase essential for type IV collagen secretion. Dev. Biol. 227:690-705. [A picture from our paper graces the cover of this issue of the journal].
6. Norman, K. and D. G. Moerman. 2002 Alpha spectrin is essential for morphogenesis and
body wall muscle formation in Caenorhabditis elegans. Journal Cell Biology 157: 665-677. [This paper was the subject of a short review in JCB]
7. Mackinnon, C., H. Qadota, K. R Norman, D. G. Moerman, and B. D. Williams. 2002 C. elegans
PAT-4/ILK functions as an adaptor protein to mediate interactions within integrin adhesion complexes. Current Biology 12:1-20. [This paper was the subject of a dispatch in Current Biology]
8. Rogalski, T.M.,, M.M. Gilbert, D. Davenport, K.R. Norman and D.G. Moerman 2003 DIM-1, a novel immunoglobulin superfamily protein in Caenorhabditis elegans necessary for maintaining body wall muscle integrity. Genetics 163: 905-915.
9. Mercer, K.B., D. B. Flaherty, R. K. Miller, H. Qadota, T.L. Tinley, D.G. Moerman, and G. M. Benian 2003 C. elegansUNC98, a C2H2 Zn finger protein, is a novel partner of UNC-97 / PINCH in muscle adhesion complexes. Mol. Biol. Cell. 14: 2492-2507.
10. Lin, X., H. Qadota, D.G. Moerman and B.D. Williams 2003 C. elegans PAT-6/Actopaxin plays a critical role in the assembly of integrin adhesion complexes in vivo. Current Biology 13: 922-932 [This paper is the subject of a dispatch in Current Biology]
11. Moerman, D. G. and Williams, B. D. Sarcomere assembly in C. elegans muscle (January 16, 2006), WormBook, ed. The C. elegans Research Community, WormBook, doi/10.1895/wormbook.1.81.1, http://www.wormbook.org.
Expression Group Publications
1. McKay, S.J., Johnsen, R. , Khattra, J., Baillie, D.L. Dube N., Fang, L., Goszczynski, B., Ha, E., Halfnight, E., Hollebakken R., Huang, P., Hung, K., Jensen, V., Jones, S.J.M., Kai, H., Li, D., Mah, A., Marra, M., McGhee, J., Newbury, R., Pouzyrev, A., Riddle D.L., Sonnhammer, E., Tian, H., Tu, D.,Tyson, J., Warner, A., Wong, K., Zhao, X.and D.G. Moerman 2004 Gene expression profiling in cells, tissues and developmental stages of the nematode C. elegans. The Genome of Homo Sapiens. Cold Spring Harbor Symposium of Quantitative Biology Volume 68: 159-169.
2. Chen, N., Pai, S., Mah, A., Zhao, Z., Newbury, R., Johnsen, R., Moerman, D.G., Baillie, D.L., and L.D. Stein, 2004 Identification of a novel nematode chemosensory gene family. In Press. Proc. Natl. Acad. Sci. USA
3. Blacque, O.E., Perens, E.A., Boroevich, K.A., Inglis, P.N., Li, C., Warner, A., Khattra, J., Holt, R.A., Ou, G., Mah, A.K., McKay, S.J., Huang, P,, Swoboda, P., Jones, S.J.M., Marra, M.A., Baillie, D.L., Moerman, D.G., Shaham, S., and Leroux, M.R. 2005 Functional genomics of the Cilium, a sensory organelle. Current Biology 15:1-7.
4. McGhee, J.D., Sleume,r M.C., Bilenky, M., Wong, K., McKay, S.J., Goszczynski, B., Tian, H., Krich, N.D., Khattra, J., Holt, R.A., Bailli,e D.L., Kohara, Y., Marra, M.A., Jones, S.J., Moerman, D.G., Robertson, A.G. 2006 The ELT-2 GATA-factor and the global regulation of transcription in the C. elegans intestine. .Dev Biol. Oct 21; [Epub ahead of print]
5. Chen, N., Mah, A., Blacque, O.E., Chu, J., Phgora, K., Bakhoum, M.W., Newbury, R.H., Khattra, J., Chan, S., Go, A., Efimenko, E., Johnsen, R., Phirke, P., Swoboda, P., Marra M., Moerman D.G., Leroux M.R., Baillie D.L., Stein L.D. 2006 Identification of ciliary and ciliopathy genes in Caenorhabditis elegans through comparative genomics. Genome Biol. 2006 Dec 22;7(12):R126 [Epub ahead of print]
6. Huang, P., E.D. Pleasance, J.S. Maydan, R. Hunt-Newbury, N.J. O’Neil, A. Mah, D.L. Baillie, M.A. Marra, D.G. Moerman, and S.J. Jones, 2007. Identification and analysis of internal promoters in Caenorhabditis elegans operons. Genome Res. 17: 1478-85.