Dr. Christopher Overall Publishes in Cell Reports

TAILS N-Terminomics and Proteomics Show Protein Degradation Dominates over Proteolytic Processing by Cathepsins in Pancreatic Tumors

Collaborator: Dr. Anna Prudova

Affiliations: Oral Biology, Centre for Blood Research, LSI

DOI: http://dx.doi.org/10.1016/j.celrep.2016.06.086

Multiplex TAILS proteomics identified substrate cleavage sites for multiple cathepsins in vivo in pancreatic cancer. Six different cathepsin knockouts were simultaneously analyzed by 8-plex iTRAQ TAILS N terminomics. The uncovered substrates suggest regulatory mechanisms for cathepsins in cancer including those driving the Warburg effect.