TAILS N-Terminomics and Proteomics Show Protein Degradation Dominates over Proteolytic Processing by Cathepsins in Pancreatic Tumors
Collaborator: Dr. Anna Prudova
Affiliations: Oral Biology, Centre for Blood Research, LSI
Multiplex TAILS proteomics identified substrate cleavage sites for multiple cathepsins in vivo in pancreatic cancer. Six different cathepsin knockouts were simultaneously analyzed by 8-plex iTRAQ TAILS N terminomics. The uncovered substrates suggest regulatory mechanisms for cathepsins in cancer including those driving the Warburg effect.